Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Crk2 around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCrk2 ( Phospho-Tyr221 ) antibody detects endogenous levels of Crk2 only when phosphorylated at tyrosine 221.
ReferencesOgawa S, et al. (1994) Oncogene 9:1669-1678.
Abassi Y.A., et al. (2002) EMBO J. 21:4571-4582.
Feller S.M., et al. (1994) EMBO J. 13:2341-2351.
Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318.
Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.
Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells