Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CHK2 around the phosphorylation site of threonine 387 (C-G-TP-P-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCHK2 (Phospho-Thr387) antibody detects endogenous levels of CHK2 only when phosphorylated at serine 317.
ReferencesGabant G, et al. (2008) J Mol Biol 380, 489-503.
Gupta SK, et al. (2007) J Biol Chem 282, 29431-40.
Oliva-Trastoy M, et al. (2007) Oncogene 26, 1449-58.
Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells