Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityASK1 (phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.
ReferencesIchijo, H. et al. (1997) Science 275, 90-94.
Wang, X.S. et al. (1996) J. Biol. Chem. 271, 31607-31611.
Matsuzawa, A. and Ichijo, H. (2001) J. Biochem. (Tokyo) 130, 1-8.
Tobiume, K. et al. (2001) EMBO Rep. 2, 222-228.
Nishitoh, H. et al. (2002) Genes Dev. 16, 1345-1355.
Zhang, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8511-8515.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells