Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human ALK around the phosphorylation site of tyrosine 1586 (Y-G-YP-Q-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityALK (phospho-Tyr1586) antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1586.
ReferencesRikova K, et al. (2007) Cell 131, 1190-203.
Stoica, G.E. et al. (2001) J. Biol. Chem. 276, 16772-16779.
Iwahara, T. et al. (1997) Oncogene 14, 439-449.
Morris, S.W. et al. (1997) Oncogene 14, 2175-2188.
Morris, S.W. et al. (1994) Science 263, 1281-1284.
Bai, R.Y. et al. (1998) Mol. Cell Biol. 18, 6951-6961.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells