Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Tsc2 around the phosphorylation site of serine 939 (S-T-SP-L-N).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityTsc2 (phospho-Ser939) antibody detects endogenous levels of Tsc2 only when phosphorylated at serine 939.
ReferencesCai SL, et al. (2006) J Cell Biol 173, 279-89.
Roux PP, et al. (2004) Proc Natl Acad Sci USA 101, 13489-94.
Li Y, Inoki K, Vacratsis P, Guan KL (2003) J Biol Chem 278, 13663-71.
Manning BD, et al. (2002) Mol Cell 10, 151-62.
Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967.
Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells