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Primary Antibodies

PDGF Receptor beta (Phospho-Tyr751) Antibody

E012033-1Cite ID: EN0001027DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
P09619
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PDGFR beta around the phosphorylation site of tyrosine 751 (V-D-YP-V-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityPDGFR beta (phospho-Tyr751) antibody detects endogenous levels of PDGFR beta only when phosphorylated at tyrosine 751.
ReferencesBergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516
Kovalenko M, et al. (2000) J Biol Chem 275, 16219-26
Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272
Wennström S, et al. (1994) Oncogene 9, 651-60
Kazlauskas A, Kashishian A, Cooper JA, Valius M (1992) Mol Cell Biol 12, 2534-44
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDGFR (phospho-Tyr751) antibody (E012033).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDGFR (phospho-Tyr751) antibody (E012033).
Western blot analysis of extracts from NIH/3T3 cells beta treated with PDGF. Left: Using PDGFR beta (phospho- Tyr751) antibody (E012033). Right: Using the same antibody preincubated with synthesized peptide.
Western blot analysis of extracts from NIH/3T3 cells beta treated with PDGF. Left: Using PDGFR beta (phospho- Tyr751) antibody (E012033). Right: Using the same antibody preincubated with synthesized peptide.

Conjugated Antibody

This product is also available conjugated. Format: E012033-1-Conjugate. Price: $485 / Size: 100μl.

E012033-1-FITC$485 / 100μlE012033-1-Biotin$485 / 100μlE012033-1-HRP$485 / 100μlE012033-1-AP$485 / 100μlE012033-1-AF350$485 / 100μlE012033-1-AF405M$485 / 100μlE012033-1-AF488$485 / 100μlE012033-1-AF514$485 / 100μlE012033-1-AF532$485 / 100μlE012033-1-AF555$485 / 100μlE012033-1-AF568$485 / 100μlE012033-1-AF405S$485 / 100μlE012033-1-AF405L$485 / 100μlE012033-1-AF546$485 / 100μlE012033-1-AF594$485 / 100μlE012033-1-AF610$485 / 100μlE012033-1-AF635$485 / 100μlE012033-1-AF647$485 / 100μlE012033-1-AF680$485 / 100μlE012033-1-AF700$485 / 100μlE012033-1-AF750$485 / 100μlE012033-1-AF790$485 / 100μlE012033-1-APC$485 / 100μlE012033-1-PE$485 / 100μlE012033-1-Cy3$485 / 100μlE012033-1-Cy5$485 / 100μlE012033-1-Cy5.5$485 / 100μlE012033-1-Cy7$485 / 100μlE012033-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.