World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
P53 (Phospho-Ser20) Antibody
$145/ 50μg/50μl
Specification
QuantityTotal: $145
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human P53 around the phosphorylation site of serine 20 (T-F-SP-D-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityP53 (phospho-Ser20) antibody detects endogenous levels of P53 only when phosphorylated at serine 20.
ReferencesWang H, et al. (2008) J Biol Chem 283, 2564-74
Mayo LD, et al. (2005) J Biol Chem 280, 25953-9
Thompson T, et al. (2004) J Biol Chem 279, 53015-22
Hirao, A. et al. (2000) Science 287, 1824-1827.
Levine, A.J. (1997) Cell 88, 323-331.
Shieh, S.Y. et al. (1997) Cell 91, 325-334.
Mayo LD, et al. (2005) J Biol Chem 280, 25953-9
Thompson T, et al. (2004) J Biol Chem 279, 53015-22
Hirao, A. et al. (2000) Science 287, 1824-1827.
Levine, A.J. (1997) Cell 88, 323-331.
Shieh, S.Y. et al. (1997) Cell 91, 325-334.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P53 (phospho- Ser20) antibody (E012030).
Western blot analysis of extracts from COS7 cells treated with UV. Left: Using P53 (phospho-Ser20) antibody (E012030). Right: Using the same antibody preincubated with synthesized peptide.
Conjugated Antibody
This product is also available conjugated. Format: E012030-1-Conjugate. Price: $485 / Size: 100μl.
E012030-1-FITC$485 / 100μlE012030-1-Biotin$485 / 100μlE012030-1-HRP$485 / 100μlE012030-1-AP$485 / 100μlE012030-1-AF350$485 / 100μlE012030-1-AF405M$485 / 100μlE012030-1-AF488$485 / 100μlE012030-1-AF514$485 / 100μlE012030-1-AF532$485 / 100μlE012030-1-AF555$485 / 100μlE012030-1-AF568$485 / 100μlE012030-1-AF405S$485 / 100μlE012030-1-AF405L$485 / 100μlE012030-1-AF546$485 / 100μlE012030-1-AF594$485 / 100μlE012030-1-AF610$485 / 100μlE012030-1-AF635$485 / 100μlE012030-1-AF647$485 / 100μlE012030-1-AF680$485 / 100μlE012030-1-AF700$485 / 100μlE012030-1-AF750$485 / 100μlE012030-1-AF790$485 / 100μlE012030-1-APC$485 / 100μlE012030-1-PE$485 / 100μlE012030-1-Cy3$485 / 100μlE012030-1-Cy5$485 / 100μlE012030-1-Cy5.5$485 / 100μlE012030-1-Cy7$485 / 100μlE012030-1-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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