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Primary Antibodies

Histone H3.1 (Phospho-Thr3) Antibody

E012022-1Cite ID: EN0001008DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
P68431
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of Threonine 3 (A-R-TP-K-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHistone H3.1 (phospho-Thr3) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at Threonine 3.
ReferencesBonenfant D, et al. (2007) Mol Cell Proteomics 6, 1917-32
Dai J, Sultan S, Taylor SS, Higgins JM (2005) Genes Dev 19, 472-88
Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45.
Cheung, P. et al. (2000) Cell 103, 263-271.
Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human malignant lymphoma tissue using Histone (phospho-Thr3) antibody (E012022).
Immunohistochemical analysis of paraffin-embedded human malignant lymphoma tissue using Histone (phospho-Thr3) antibody (E012022).
Western blot analysis of extracts from HuvEc cells H3.1 treated with Serum. Left: Using Histone H3.1 (phospho-Thr3) antibody (E012022). Right: Using the same antibody preincubated with synthesized peptide.
Western blot analysis of extracts from HuvEc cells H3.1 treated with Serum. Left: Using Histone H3.1 (phospho-Thr3) antibody (E012022). Right: Using the same antibody preincubated with synthesized peptide.

Conjugated Antibody

This product is also available conjugated. Format: E012022-1-Conjugate. Price: $485 / Size: 100μl.

E012022-1-FITC$485 / 100μlE012022-1-Biotin$485 / 100μlE012022-1-HRP$485 / 100μlE012022-1-AP$485 / 100μlE012022-1-AF350$485 / 100μlE012022-1-AF405M$485 / 100μlE012022-1-AF488$485 / 100μlE012022-1-AF514$485 / 100μlE012022-1-AF532$485 / 100μlE012022-1-AF555$485 / 100μlE012022-1-AF568$485 / 100μlE012022-1-AF405S$485 / 100μlE012022-1-AF405L$485 / 100μlE012022-1-AF546$485 / 100μlE012022-1-AF594$485 / 100μlE012022-1-AF610$485 / 100μlE012022-1-AF635$485 / 100μlE012022-1-AF647$485 / 100μlE012022-1-AF680$485 / 100μlE012022-1-AF700$485 / 100μlE012022-1-AF750$485 / 100μlE012022-1-AF790$485 / 100μlE012022-1-APC$485 / 100μlE012022-1-PE$485 / 100μlE012022-1-Cy3$485 / 100μlE012022-1-Cy5$485 / 100μlE012022-1-Cy5.5$485 / 100μlE012022-1-Cy7$485 / 100μlE012022-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.