Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human FOXO3A around the phosphorylation site of serine 253 (A-V-SP-M-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityFOXO3A (phospho-Ser253) antibody detects endogenous levels of FOXO3A only when phosphorylated at serine 253.
ReferencesLi M, Chiu JF, Mossman BT, Fukagawa NK (2006) D J Biol Chem 281, 40429-39
Essafi A, et al. (2005) Oncogene 24, 2317-29
Seoane, J. et al. (2004) Cell 117, 211-223.
Brunet A, et al. (2001) Mol Cell Biol 21, 952-65
Brunet A, et al. (1999) Cell 96, 857-68
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells