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Primary Antibodies

FOXO3A (Phospho-Ser253) Antibody

E012020-1Cite ID: EN0001004DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
O43524
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human FOXO3A around the phosphorylation site of serine 253 (A-V-SP-M-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityFOXO3A (phospho-Ser253) antibody detects endogenous levels of FOXO3A only when phosphorylated at serine 253.
ReferencesLi M, Chiu JF, Mossman BT, Fukagawa NK (2006) D J Biol Chem 281, 40429-39
Essafi A, et al. (2005) Oncogene 24, 2317-29
Seoane, J. et al. (2004) Cell 117, 211-223.
Brunet A, et al. (2001) Mol Cell Biol 21, 952-65
Brunet A, et al. (1999) Cell 96, 857-68
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3A (phospho- Ser253) antibody (E012020).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3A (phospho- Ser253) antibody (E012020).
Western blot analysis of extracts from NIH/3T3 cells treated with using Serum. Left: Using FOXO3A (phospho-Ser253) antibody (E012020). Right: Using the same antibody preincubated with synthesized peptide.
Western blot analysis of extracts from NIH/3T3 cells treated with using Serum. Left: Using FOXO3A (phospho-Ser253) antibody (E012020). Right: Using the same antibody preincubated with synthesized peptide.

Conjugated Antibody

This product is also available conjugated. Format: E012020-1-Conjugate. Price: $485 / Size: 100μl.

E012020-1-FITC$485 / 100μlE012020-1-Biotin$485 / 100μlE012020-1-HRP$485 / 100μlE012020-1-AP$485 / 100μlE012020-1-AF350$485 / 100μlE012020-1-AF405M$485 / 100μlE012020-1-AF488$485 / 100μlE012020-1-AF514$485 / 100μlE012020-1-AF532$485 / 100μlE012020-1-AF555$485 / 100μlE012020-1-AF568$485 / 100μlE012020-1-AF405S$485 / 100μlE012020-1-AF405L$485 / 100μlE012020-1-AF546$485 / 100μlE012020-1-AF594$485 / 100μlE012020-1-AF610$485 / 100μlE012020-1-AF635$485 / 100μlE012020-1-AF647$485 / 100μlE012020-1-AF680$485 / 100μlE012020-1-AF700$485 / 100μlE012020-1-AF750$485 / 100μlE012020-1-AF790$485 / 100μlE012020-1-APC$485 / 100μlE012020-1-PE$485 / 100μlE012020-1-Cy3$485 / 100μlE012020-1-Cy5$485 / 100μlE012020-1-Cy5.5$485 / 100μlE012020-1-Cy7$485 / 100μlE012020-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

FOXO3A (Phospho-Ser253) Antibody | EnoGene Biotech Co. Ltd