Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPKbeta1 around the phosphorylation site of serine 182 (S-S-SP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityAMPKbeta1 (phospho-Ser182) antibody detects endogenous levels of AMPKbeta1 only when phosphorylated at serine 182.
ReferencesWarden, S.M. et al. (2001) Biochem. J. 354, 275-283.
Yin W, Mu J, Birnbaum MJ (2003) J Biol Chem 278, 43074-80
Hardie, D.G. (2004) J. Cell Sci. 117, 5479-5487.
Shaw, R. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335.
Watt MJ, et al. (2006) Am J Physiol Endocrinol Metab 290, E500-8
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells