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Primary Antibodies

AMPKbeta1 (Phospho-Ser182) Antibody

E012004-1Cite ID: EN0000984DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Q9Y478
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPKbeta1 around the phosphorylation site of serine 182 (S-S-SP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAMPKbeta1 (phospho-Ser182) antibody detects endogenous levels of AMPKbeta1 only when phosphorylated at serine 182.
ReferencesWarden, S.M. et al. (2001) Biochem. J. 354, 275-283.
Yin W, Mu J, Birnbaum MJ (2003) J Biol Chem 278, 43074-80
Hardie, D.G. (2004) J. Cell Sci. 117, 5479-5487.
Shaw, R. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335.
Watt MJ, et al. (2006) Am J Physiol Endocrinol Metab 290, E500-8
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AMPKbeta1 (phospho-Ser182) (E012004).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AMPKbeta1 (phospho-Ser182) (E012004).
Western blot analysis of extracts from Jurkat cells. Left: Using AMPKbeta1 (phospho-Ser182) antibody (E012004). Right: Using the same antibody preincubated with synthesized peptide.
Western blot analysis of extracts from Jurkat cells. Left: Using AMPKbeta1 (phospho-Ser182) antibody (E012004). Right: Using the same antibody preincubated with synthesized peptide.

Conjugated Antibody

This product is also available conjugated. Format: E012004-1-Conjugate. Price: $485 / Size: 100μl.

E012004-1-FITC$485 / 100μlE012004-1-Biotin$485 / 100μlE012004-1-HRP$485 / 100μlE012004-1-AP$485 / 100μlE012004-1-AF350$485 / 100μlE012004-1-AF405M$485 / 100μlE012004-1-AF488$485 / 100μlE012004-1-AF514$485 / 100μlE012004-1-AF532$485 / 100μlE012004-1-AF555$485 / 100μlE012004-1-AF568$485 / 100μlE012004-1-AF405S$485 / 100μlE012004-1-AF405L$485 / 100μlE012004-1-AF546$485 / 100μlE012004-1-AF594$485 / 100μlE012004-1-AF610$485 / 100μlE012004-1-AF635$485 / 100μlE012004-1-AF647$485 / 100μlE012004-1-AF680$485 / 100μlE012004-1-AF700$485 / 100μlE012004-1-AF750$485 / 100μlE012004-1-AF790$485 / 100μlE012004-1-APC$485 / 100μlE012004-1-PE$485 / 100μlE012004-1-Cy3$485 / 100μlE012004-1-Cy5$485 / 100μlE012004-1-Cy5.5$485 / 100μlE012004-1-Cy7$485 / 100μlE012004-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.