Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Abl1 around the phosphorylation site of tyrosine 412 (D-T-YP-T-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityAbl1 (phospho-Tyr412) antibody detects endogenous levels of Abl1 only when phosphorylated at tyrosine 412.
ReferencesWang J.Y. et al. (2000) Oncogene 19, 5643-5650.
Danial N.N. et al. (2000) Oncogene 19, 2523-2531.
Pluk H. et al. (2002) Cell 108, 247-259.
Rikova K, et al. (2007) Cell 131, 1190-203.
Goss VL, et al. (2006) Blood 107, 4888-97.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells