Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 65 (R-N-SP-P-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity4E-BP1 (phospho-Ser65) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at serine 65.
ReferencesPause A. et al. (1994) Nature 371, 762-767.
Brunn G.J. et al. (1997) Science 277, 99-101.
Gingras AC, et al. (2001) Genes Dev 15, 2852-64.
Lal L, et al. (2005) Blood 105, 1669-77.
Molina H, et al. (2007) Proc Natl Acad Sci USA 104, 2199-204.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells