Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human
Cyclin E1 around the phosphorylation site of threonine395 (L-L-TP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCyclin E1 (phospho-Thr395) Antibody detects endogenous levels of Cyclin E1only when
phosphorylated at threonine395.
ReferencesLauper, N. et al. (1998) Oncogene 17, 2637-2643.
Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193.
Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells