Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human
AKT1/AKT2 around the phosphorylation site of Threonine 308/309 (M-K-TP-F-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityAKT1/AKT2 (phospho-Thr308/309) Antibody detects endogenous levels of AKT1/AKT2 only
when phosphorylated at Threonine 308/309.
ReferencesFeng J, et al. (2004) J Biol Chem; 279(34): 35510-7.
Ayala G, et al. (2004) Clin Cancer Res; 10(19): 6572-8.
Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.
Min YH, et al. (2004) Cancer Res; 64(15): 5225-31.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells