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Primary Antibodies

AKT1 (phospho-Thr450) Antibody

E011502-1Cite ID: EN0000600DatasheetCOAMSDS
Human, Mouse, Rat
IHC: 1:50~100 IF:1:100~1:200
P31749
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAKT1 (phospho-Thr450) Antibody detects endogenous levels of AKT1 only when phosphorylated at threonine 450.
ReferencesFranke, T.F. et al. (1997) Cell 88, 435-7.
Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (phospho- Thr450) Antibody (E011502).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (phospho- Thr450) Antibody (E011502).
Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450) Antibody (E011502, red).
Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450) Antibody (E011502, red).

Conjugated Antibody

This product is also available conjugated. Format: E011502-1-Conjugate. Price: $485 / Size: 100μl.

E011502-1-FITC$485 / 100μlE011502-1-Biotin$485 / 100μlE011502-1-HRP$485 / 100μlE011502-1-AP$485 / 100μlE011502-1-AF350$485 / 100μlE011502-1-AF405M$485 / 100μlE011502-1-AF488$485 / 100μlE011502-1-AF514$485 / 100μlE011502-1-AF532$485 / 100μlE011502-1-AF555$485 / 100μlE011502-1-AF568$485 / 100μlE011502-1-AF405S$485 / 100μlE011502-1-AF405L$485 / 100μlE011502-1-AF546$485 / 100μlE011502-1-AF594$485 / 100μlE011502-1-AF610$485 / 100μlE011502-1-AF635$485 / 100μlE011502-1-AF647$485 / 100μlE011502-1-AF680$485 / 100μlE011502-1-AF700$485 / 100μlE011502-1-AF750$485 / 100μlE011502-1-AF790$485 / 100μlE011502-1-APC$485 / 100μlE011502-1-PE$485 / 100μlE011502-1-Cy3$485 / 100μlE011502-1-Cy5$485 / 100μlE011502-1-Cy5.5$485 / 100μlE011502-1-Cy7$485 / 100μlE011502-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

AKT1 (phospho-Thr450) Antibody | EnoGene Biotech Co. Ltd