Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CARM1 around the phosphorylation site of serine 228 (V-K-SP-N-N).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCARM1 (Phospho-Ser228) antibody detects endogenous levels of CARM1 only when phosphorylated at serine 228.
ReferencesSelma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103(36): 13351–13356.
Marcela Covic, et al. (2005) EMBO J; 24(1): 85–96.
Eleni Zika et al. (2005) Proc Natl Acad Sci U S A.; 102(45): 16321–16326
Neelu Yadav et al. (2003) Proc Natl Acad Sci U S A; 100(11): 6464–6468
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells