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Primary Antibodies

Smad2 (Phospho-Thr220) Antibody

E011323-1Cite ID: EN0000580DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000
Q15796
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivitySmad2 (Phospho-Thr220) Antibody detects endogenous levels of Smad2 only when phosphorylated at threonine 220.
ReferencesSang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420–434.
Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24(3): 1106–1121.
Kimberly A Brown et al. (2004) Breast Cancer Res.; 6(3): R215–R231.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extract from Hela cells untreated or treated with IL-4 (100ng/mL, 30min), using Smad2 (Ab-220) Antibody (E021323, Lane 1 and 2) and Smad2 (Phospho- Thr220) Antibody (E011323, Lane 3 and 4).
Western blot analysis of extract from Hela cells untreated or treated with IL-4 (100ng/mL, 30min), using Smad2 (Ab-220) Antibody (E021323, Lane 1 and 2) and Smad2 (Phospho- Thr220) Antibody (E011323, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011323-1-Conjugate. Price: $485 / Size: 100μl.

E011323-1-FITC$485 / 100μlE011323-1-Biotin$485 / 100μlE011323-1-HRP$485 / 100μlE011323-1-AP$485 / 100μlE011323-1-AF350$485 / 100μlE011323-1-AF405M$485 / 100μlE011323-1-AF488$485 / 100μlE011323-1-AF514$485 / 100μlE011323-1-AF532$485 / 100μlE011323-1-AF555$485 / 100μlE011323-1-AF568$485 / 100μlE011323-1-AF405S$485 / 100μlE011323-1-AF405L$485 / 100μlE011323-1-AF546$485 / 100μlE011323-1-AF594$485 / 100μlE011323-1-AF610$485 / 100μlE011323-1-AF635$485 / 100μlE011323-1-AF647$485 / 100μlE011323-1-AF680$485 / 100μlE011323-1-AF700$485 / 100μlE011323-1-AF750$485 / 100μlE011323-1-AF790$485 / 100μlE011323-1-APC$485 / 100μlE011323-1-PE$485 / 100μlE011323-1-Cy3$485 / 100μlE011323-1-Cy5$485 / 100μlE011323-1-Cy5.5$485 / 100μlE011323-1-Cy7$485 / 100μlE011323-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.