Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-SP).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivitySmad2 (Phospho-Ser467) Antibody detects endogenous levels of Smad2 only when phosphorylated at serine 467.
ReferencesSang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420–434.
Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24(3): 1106–1121.
Kimberly A Brown et al. (2004) Breast Cancer Res.; 6(3): R215–R231.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells