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Primary Antibodies

Smad1 (Phospho-Ser465) Antibody

E011321-1Cite ID: EN0000576DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000
Q15797
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivitySmad1 (Phospho-Ser465) Antibody detects endogenous levels of Smad1 only when phosphorylated at serine 465.
ReferencesHiroaki Seto, et al. (2004) J Clin Invest. March 1; 113(5): 718–726
Linyu Li, et al. (2004) Mol Cell Biol. January; 24(2): 856–864.
Claire Nourry, et al. (2004) BMC Cell Biol. 5: 20
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extract from 293 cells, untreated or treated with PMA (200nM, 30min), using Smad1 (Ab-465) Antibody (E021321, Lane 1 and 2) and Smad1 (Phospho-Ser465) Antibody (E011321, Lane 3 and 4).
Western blot analysis of extract from 293 cells, untreated or treated with PMA (200nM, 30min), using Smad1 (Ab-465) Antibody (E021321, Lane 1 and 2) and Smad1 (Phospho-Ser465) Antibody (E011321, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011321-1-Conjugate. Price: $485 / Size: 100μl.

E011321-1-FITC$485 / 100μlE011321-1-Biotin$485 / 100μlE011321-1-HRP$485 / 100μlE011321-1-AP$485 / 100μlE011321-1-AF350$485 / 100μlE011321-1-AF405M$485 / 100μlE011321-1-AF488$485 / 100μlE011321-1-AF514$485 / 100μlE011321-1-AF532$485 / 100μlE011321-1-AF555$485 / 100μlE011321-1-AF568$485 / 100μlE011321-1-AF405S$485 / 100μlE011321-1-AF405L$485 / 100μlE011321-1-AF546$485 / 100μlE011321-1-AF594$485 / 100μlE011321-1-AF610$485 / 100μlE011321-1-AF635$485 / 100μlE011321-1-AF647$485 / 100μlE011321-1-AF680$485 / 100μlE011321-1-AF700$485 / 100μlE011321-1-AF750$485 / 100μlE011321-1-AF790$485 / 100μlE011321-1-APC$485 / 100μlE011321-1-PE$485 / 100μlE011321-1-Cy3$485 / 100μlE011321-1-Cy5$485 / 100μlE011321-1-Cy5.5$485 / 100μlE011321-1-Cy7$485 / 100μlE011321-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.