Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human
Keratin 8 around the phosphorylation site of serine 74 (L-L-SP-P-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityKeratin 8 (Phospho-Ser74) Antibody detects endogenous levels of Keratin 8 only when
phosphorylated at serine 74.
ReferencesNakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451.
Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322.
Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877.
Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells