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Primary Antibodies

GSK3β (Phospho-Tyr216) Antibody

E011301-1Cite ID: EN0000543DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50-1:100
P49841
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human GSK3β around the phosphorylation site of tyrosine 216 (V-S-YP-I-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityGSK3β (Phospho-Tyr216) antibody detects endogenous levels of GSK3β only when phosphorylated at tyrosine 216.
ReferencesChin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137(1-2): 193-201
Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316(2): 411-415
Fan G, et al. J Biol Chem 2003 Dec 26; 278(52): 52432-52436
Liao X, et al. Mol Cancer Ther 2003 Nov; 2(11): 1215-1222
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK3β (Phospho-Tyr216) antibody (E011301).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK3β (Phospho-Tyr216) antibody (E011301).
Western blot analysis of extract from 293 cells, untreated or treated with insulin (40µM, 30min), using GSK3β (Ab-216) Antibody (E021301, Lane 1 and 2) and GSK3β (Phospho-Tyr216) Antibody (E011301, Lane 3 and 4).
Western blot analysis of extract from 293 cells, untreated or treated with insulin (40µM, 30min), using GSK3β (Ab-216) Antibody (E021301, Lane 1 and 2) and GSK3β (Phospho-Tyr216) Antibody (E011301, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011301-1-Conjugate. Price: $485 / Size: 100μl.

E011301-1-FITC$485 / 100μlE011301-1-Biotin$485 / 100μlE011301-1-HRP$485 / 100μlE011301-1-AP$485 / 100μlE011301-1-AF350$485 / 100μlE011301-1-AF405M$485 / 100μlE011301-1-AF488$485 / 100μlE011301-1-AF514$485 / 100μlE011301-1-AF532$485 / 100μlE011301-1-AF555$485 / 100μlE011301-1-AF568$485 / 100μlE011301-1-AF405S$485 / 100μlE011301-1-AF405L$485 / 100μlE011301-1-AF546$485 / 100μlE011301-1-AF594$485 / 100μlE011301-1-AF610$485 / 100μlE011301-1-AF635$485 / 100μlE011301-1-AF647$485 / 100μlE011301-1-AF680$485 / 100μlE011301-1-AF700$485 / 100μlE011301-1-AF750$485 / 100μlE011301-1-AF790$485 / 100μlE011301-1-APC$485 / 100μlE011301-1-PE$485 / 100μlE011301-1-Cy3$485 / 100μlE011301-1-Cy5$485 / 100μlE011301-1-Cy5.5$485 / 100μlE011301-1-Cy7$485 / 100μlE011301-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.