Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from mouse
BIM around the phosphorylation site of serine 65 (P-A-SP-P-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityBIM (phospho-Ser65) antibody detects endogenous levels of BIM only when phosphorylated at serine 65.
ReferencesFukazawa H, et al. (2004) Mol Cancer Ther ; 3(10): 1281-1288
Luciano F, et al. (2003) Oncogene ; 22(43): 6785-6793
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells