Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CaMKII around the phosphorylation site of threonine 286 (Q-E-TP-V-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCaMKII (phospho-Thr286) antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 286.
ReferencesPak JH, et al. Proc Natl Acad Sci U S A. 2000 Oct 10; 97(21): 11232-11237
Hudmon A, et al. J Cell Biol. Author manuscript; available in PMC 2006 May 7
Miller P, et al. PLoS Biol. 2005 Apr; 3(4): e107
Runyan JD, et al. Learn Mem. 2005 Mar; 12(2): 103-110.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells