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Primary Antibodies

p70 S6 Kinase (Phospho-Ser424) Antibody

E011284-1Cite ID: EN0000517DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50-1:100 IF: 1:100-200
P23443
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-SP-P-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivityp70 S6 Kinase (phospho-Ser424) antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at serine 424.
ReferencesSatoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484–97
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (phospho-Ser424) antibody (E011284).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (phospho-Ser424) antibody (E011284).
Western blot analysis of extracts from 293 cells, untreated Western blot analysis of extracts from 293 cells untreated or treated with serum for the indicated times, using p70 S6 Kinase (phospho-Ser424) antibody (E011284).
Western blot analysis of extracts from 293 cells, untreated Western blot analysis of extracts from 293 cells untreated or treated with serum for the indicated times, using p70 S6 Kinase (phospho-Ser424) antibody (E011284).

Conjugated Antibody

This product is also available conjugated. Format: E011284-1-Conjugate. Price: $485 / Size: 100μl.

E011284-1-FITC$485 / 100μlE011284-1-Biotin$485 / 100μlE011284-1-HRP$485 / 100μlE011284-1-AP$485 / 100μlE011284-1-AF350$485 / 100μlE011284-1-AF405M$485 / 100μlE011284-1-AF488$485 / 100μlE011284-1-AF514$485 / 100μlE011284-1-AF532$485 / 100μlE011284-1-AF555$485 / 100μlE011284-1-AF568$485 / 100μlE011284-1-AF405S$485 / 100μlE011284-1-AF405L$485 / 100μlE011284-1-AF546$485 / 100μlE011284-1-AF594$485 / 100μlE011284-1-AF610$485 / 100μlE011284-1-AF635$485 / 100μlE011284-1-AF647$485 / 100μlE011284-1-AF680$485 / 100μlE011284-1-AF700$485 / 100μlE011284-1-AF750$485 / 100μlE011284-1-AF790$485 / 100μlE011284-1-APC$485 / 100μlE011284-1-PE$485 / 100μlE011284-1-Cy3$485 / 100μlE011284-1-Cy5$485 / 100μlE011284-1-Cy5.5$485 / 100μlE011284-1-Cy7$485 / 100μlE011284-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.