Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 446 (K-R-TP-R-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityPKR (phospho-Thr446) antibody detects endogenous levels of PKR only when phosphorylated at threonine 446.
ReferencesAbujiang Pataer, et,al. (2002) Cancer Res; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 - 18745.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells