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Primary Antibodies

eIF2α(Phospho-Ser51) Antibody

E011279-1Cite ID: EN0000509DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
P05198
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human eIF2α around the phosphorylation site of serine 51 (E-L-SP-R-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityeIF2α (phospho-Ser51) antibody detects endogenous levels of eIF2α only when phosphorylated at serine 51.
ReferencesXavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using eIF2α (phospho-Ser51) antibody (E011279).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using eIF2α (phospho-Ser51) antibody (E011279).
Western blot analysis of extracts from K562 cells untreated or treated with IFN-α (100ng/ml, 20min), using eIF2α (Ab-51) antibody (E021271, Lane 1 and 2) and eIF2α (phospho-Ser51) antibody (E011279, Lane 3 and 4).
Western blot analysis of extracts from K562 cells untreated or treated with IFN-α (100ng/ml, 20min), using eIF2α (Ab-51) antibody (E021271, Lane 1 and 2) and eIF2α (phospho-Ser51) antibody (E011279, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011279-1-Conjugate. Price: $485 / Size: 100μl.

E011279-1-FITC$485 / 100μlE011279-1-Biotin$485 / 100μlE011279-1-HRP$485 / 100μlE011279-1-AP$485 / 100μlE011279-1-AF350$485 / 100μlE011279-1-AF405M$485 / 100μlE011279-1-AF488$485 / 100μlE011279-1-AF514$485 / 100μlE011279-1-AF532$485 / 100μlE011279-1-AF555$485 / 100μlE011279-1-AF568$485 / 100μlE011279-1-AF405S$485 / 100μlE011279-1-AF405L$485 / 100μlE011279-1-AF546$485 / 100μlE011279-1-AF594$485 / 100μlE011279-1-AF610$485 / 100μlE011279-1-AF635$485 / 100μlE011279-1-AF647$485 / 100μlE011279-1-AF680$485 / 100μlE011279-1-AF700$485 / 100μlE011279-1-AF750$485 / 100μlE011279-1-AF790$485 / 100μlE011279-1-APC$485 / 100μlE011279-1-PE$485 / 100μlE011279-1-Cy3$485 / 100μlE011279-1-Cy5$485 / 100μlE011279-1-Cy5.5$485 / 100μlE011279-1-Cy7$485 / 100μlE011279-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.