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Primary Antibodies

Cortactin(Phospho-Tyr466) Antibody

E011272-1Cite ID: EN0000499DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000
Q14247
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCortactin (Phospho-Tyr466) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.
ReferencesLuo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
Li Y, et al. (2000) J Biol Chem ; 275(47): 37187-37193
Kinley AW, et al. (2003) Curr Biol ; 13(5): 384-393
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin (Ab-466) antibody (E021264, Lane 1 and 2) and Cortactin (Phospho-Tyr466) antibody (E011272, Lane 3 and 4).
Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin (Ab-466) antibody (E021264, Lane 1 and 2) and Cortactin (Phospho-Tyr466) antibody (E011272, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011272-1-Conjugate. Price: $485 / Size: 100μl.

E011272-1-FITC$485 / 100μlE011272-1-Biotin$485 / 100μlE011272-1-HRP$485 / 100μlE011272-1-AP$485 / 100μlE011272-1-AF350$485 / 100μlE011272-1-AF405M$485 / 100μlE011272-1-AF488$485 / 100μlE011272-1-AF514$485 / 100μlE011272-1-AF532$485 / 100μlE011272-1-AF555$485 / 100μlE011272-1-AF568$485 / 100μlE011272-1-AF405S$485 / 100μlE011272-1-AF405L$485 / 100μlE011272-1-AF546$485 / 100μlE011272-1-AF594$485 / 100μlE011272-1-AF610$485 / 100μlE011272-1-AF635$485 / 100μlE011272-1-AF647$485 / 100μlE011272-1-AF680$485 / 100μlE011272-1-AF700$485 / 100μlE011272-1-AF750$485 / 100μlE011272-1-AF790$485 / 100μlE011272-1-APC$485 / 100μlE011272-1-PE$485 / 100μlE011272-1-Cy3$485 / 100μlE011272-1-Cy5$485 / 100μlE011272-1-Cy5.5$485 / 100μlE011272-1-Cy7$485 / 100μlE011272-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.