Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 303 (P-P-SP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityHSF1 (phospho-Ser303) antibody detects endogenous levels of HSF1 only when phosphorylated at serine 303.
ReferencesParvaneh Rafiee, et,al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945
Fumika Shinozaki, et,al. (2006) J. Biol. Chem ; 281: 16361 - 16369.
Eiichi Takaki, et,al. (2006) J. Biol. Chem ; 281: 4931 - 4937.
Jan-Jong Hung, et,al. (1998) J. Biol. Chem ; 273: 31924.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells