Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human NFκB-p105/p50 around the phosphorylation site of serine 932 (E-T-SP-F-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityNFκB-p105/p50 (phospho-Ser932) antibody detects endogenous levels of NFκB-p105/p50 only when phosphorylated at serine 932.
ReferencesSören Beinke et al. (2004) Biochem J. 382(Pt 2): 393–409.
Vigo Heissmeyer1, et al. (1999) The EMBO Journal 18: 4766–4778,
F Chen, et al. (2006) Cell Death and Differentiation 13: 1835–1838.
Kris A. Steinbrecher, et al. (2005) Mol Cell Biol. 25(19): 8444–8455.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells