Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityHSP27 (phospho-Ser78) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 78.
ReferencesJ Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803
Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301
Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003)
Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells