World supplier of antibodies, antigens and kits since 2007
Primary Antibodies

HSP27(Phospho-Ser78) Antibody

E011247-1Cite ID: EN0000456DatasheetCOAMSDS
Human,
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
P04792
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHSP27 (phospho-Ser78) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 78.
ReferencesJ Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803
Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301
Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003)
Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 (phospho- Ser78) antibody (E011247).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 (phospho- Ser78) antibody (E011247).
Western blot analysis of extracts from HeLa cells using HSP27 (Ab-78) antibody (E021239, Lane 1 and 2) and HSP27 (phospho-Ser78) antibody (E011247, Lane 3 and 4).
Western blot analysis of extracts from HeLa cells using HSP27 (Ab-78) antibody (E021239, Lane 1 and 2) and HSP27 (phospho-Ser78) antibody (E011247, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011247-1-Conjugate. Price: $485 / Size: 100μl.

E011247-1-FITC$485 / 100μlE011247-1-Biotin$485 / 100μlE011247-1-HRP$485 / 100μlE011247-1-AP$485 / 100μlE011247-1-AF350$485 / 100μlE011247-1-AF405M$485 / 100μlE011247-1-AF488$485 / 100μlE011247-1-AF514$485 / 100μlE011247-1-AF532$485 / 100μlE011247-1-AF555$485 / 100μlE011247-1-AF568$485 / 100μlE011247-1-AF405S$485 / 100μlE011247-1-AF405L$485 / 100μlE011247-1-AF546$485 / 100μlE011247-1-AF594$485 / 100μlE011247-1-AF610$485 / 100μlE011247-1-AF635$485 / 100μlE011247-1-AF647$485 / 100μlE011247-1-AF680$485 / 100μlE011247-1-AF700$485 / 100μlE011247-1-AF750$485 / 100μlE011247-1-AF790$485 / 100μlE011247-1-APC$485 / 100μlE011247-1-PE$485 / 100μlE011247-1-Cy3$485 / 100μlE011247-1-Cy5$485 / 100μlE011247-1-Cy5.5$485 / 100μlE011247-1-Cy7$485 / 100μlE011247-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.