Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCDC2 (phospho-Tyr15) antibody detects endogenous levels of CDC2 only when phosphorylated at tyrosine 15.
ReferencesY Gu, et al. (1992) EMBO J. 11(11): 3995–4005.
P Jin, et al. (1996) Cell Biology, Vol 134: 963-970,
Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987(10)
Xiang S. et al. (1997) The EMBO Journal 16: 182–192
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells