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Primary Antibodies

CDC2 (Phospho-Tyr15) Antibody

E011244-1Cite ID: EN0000450DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000
P06493
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCDC2 (phospho-Tyr15) antibody detects endogenous levels of CDC2 only when phosphorylated at tyrosine 15.
ReferencesY Gu, et al. (1992) EMBO J. 11(11): 3995–4005.
P Jin, et al. (1996) Cell Biology, Vol 134: 963-970,
Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987(10)
Xiang S. et al. (1997) The EMBO Journal 16: 182–192
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from COLO (Lane 1, 5) and HepG2 (Lane 2, 6) and K562 (Lane 3, 4, 7, 8) cells, using CDC2 (Ab-15) antibody (E021236, Lane 1, 2, 3 and 4) and CDC2 (phospho-Tyr15) antibody (E011244, Lane 5, 6, 7 and 8).
Western blot analysis of extracts from COLO (Lane 1, 5) and HepG2 (Lane 2, 6) and K562 (Lane 3, 4, 7, 8) cells, using CDC2 (Ab-15) antibody (E021236, Lane 1, 2, 3 and 4) and CDC2 (phospho-Tyr15) antibody (E011244, Lane 5, 6, 7 and 8).

Conjugated Antibody

This product is also available conjugated. Format: E011244-1-Conjugate. Price: $485 / Size: 100μl.

E011244-1-FITC$485 / 100μlE011244-1-Biotin$485 / 100μlE011244-1-HRP$485 / 100μlE011244-1-AP$485 / 100μlE011244-1-AF350$485 / 100μlE011244-1-AF405M$485 / 100μlE011244-1-AF488$485 / 100μlE011244-1-AF514$485 / 100μlE011244-1-AF532$485 / 100μlE011244-1-AF555$485 / 100μlE011244-1-AF568$485 / 100μlE011244-1-AF405S$485 / 100μlE011244-1-AF405L$485 / 100μlE011244-1-AF546$485 / 100μlE011244-1-AF594$485 / 100μlE011244-1-AF610$485 / 100μlE011244-1-AF635$485 / 100μlE011244-1-AF647$485 / 100μlE011244-1-AF680$485 / 100μlE011244-1-AF700$485 / 100μlE011244-1-AF750$485 / 100μlE011244-1-AF790$485 / 100μlE011244-1-APC$485 / 100μlE011244-1-PE$485 / 100μlE011244-1-Cy3$485 / 100μlE011244-1-Cy5$485 / 100μlE011244-1-Cy5.5$485 / 100μlE011244-1-Cy7$485 / 100μlE011244-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.