Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human c-kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivityc-Kit(Phospho-Tyr721) antibody detects endogenous levels of c-kit only when phosphorylated at tyrosine 721.
ReferencesNocka, K. et al. (1990) EMBO J. 9, 1805-1813.
Hirota, S. et al. (1998) Science 279, 577-580.
Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells