Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 307 (T-E-SP-I-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIRS-1 (phospho-Ser307) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 307.
ReferencesSteppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575
Tuncman G, et al. (2006) Proc Natl Acad Sci U S A; 103(28): 10741-10746
Shoelson SE, et al. (2006) J Clin Invest; 116(7): 1793-1801
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells