Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityelF4E (phospho-Ser209) antibody detects endogenous levels of elF4E only when phosphorylated at serine 209.
ReferencesLi BD, et al. (1998) Ann Surg; 227(5): 756-763
Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520
De Gregorio E, et al. (2001) RNA; 7(1): 106-113
Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells