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Primary Antibodies

S6 Ribosomal Protein (Phospho-Ser235) Antibody

E011232-1Cite ID: EN0000433DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000
P62753
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

Altername40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6, ribosomal protein S6
All SitesHuman: Ser235; Mouse: Ser235; Rat: Ser235

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityS6 Ribosomal protein (phospho-Ser235) antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at serine 235.
ReferencesMcBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081.
Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097.
Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222.
Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min), using S6 Ribosomal protein (Ab-235) antibody (E021225, Line 1 and 2) and S6 Ribosomal protein (phospho-Ser235) antibody (E011232, Line 3 and 4).
Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min), using S6 Ribosomal protein (Ab-235) antibody (E021225, Line 1 and 2) and S6 Ribosomal protein (phospho-Ser235) antibody (E011232, Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011232-1-Conjugate. Price: $485 / Size: 100μl.

E011232-1-FITC$485 / 100μlE011232-1-Biotin$485 / 100μlE011232-1-HRP$485 / 100μlE011232-1-AP$485 / 100μlE011232-1-AF350$485 / 100μlE011232-1-AF405M$485 / 100μlE011232-1-AF488$485 / 100μlE011232-1-AF514$485 / 100μlE011232-1-AF532$485 / 100μlE011232-1-AF555$485 / 100μlE011232-1-AF568$485 / 100μlE011232-1-AF405S$485 / 100μlE011232-1-AF405L$485 / 100μlE011232-1-AF546$485 / 100μlE011232-1-AF594$485 / 100μlE011232-1-AF610$485 / 100μlE011232-1-AF635$485 / 100μlE011232-1-AF647$485 / 100μlE011232-1-AF680$485 / 100μlE011232-1-AF700$485 / 100μlE011232-1-AF750$485 / 100μlE011232-1-AF790$485 / 100μlE011232-1-APC$485 / 100μlE011232-1-PE$485 / 100μlE011232-1-Cy3$485 / 100μlE011232-1-Cy5$485 / 100μlE011232-1-Cy5.5$485 / 100μlE011232-1-Cy7$485 / 100μlE011232-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.