Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-SP-V-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIRS-1 (phospho-Ser639) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 639.
ReferencesOzes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Steppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575
Batty IH, et al. (2004) Biochem J; 379(Pt 3): 641-651
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells