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Primary Antibodies

IRS-1(Phospho-Ser636) Antibody

E011230-1Cite ID: EN0000429DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50-1:100
P35568
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 (P-M-SP-P-K).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityIRS-1 (phospho-Ser636) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 636.
ReferencesOzes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (phospho- Ser636) antibody (E011230).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (phospho- Ser636) antibody (E011230).
Western blot analysis of extracts from 293 cells treated with insulin(100nM, 30min) or PMA (0.2μM, 15min) using IRS-1 (Ab-636) antibody (E021223, Lane 1, 2 and 3) and IRS-1 (phospho-Ser636) antibody (E011230, Lane 4, 5 and 6).
Western blot analysis of extracts from 293 cells treated with insulin(100nM, 30min) or PMA (0.2μM, 15min) using IRS-1 (Ab-636) antibody (E021223, Lane 1, 2 and 3) and IRS-1 (phospho-Ser636) antibody (E011230, Lane 4, 5 and 6).

Conjugated Antibody

This product is also available conjugated. Format: E011230-1-Conjugate. Price: $485 / Size: 100μl.

E011230-1-FITC$485 / 100μlE011230-1-Biotin$485 / 100μlE011230-1-HRP$485 / 100μlE011230-1-AP$485 / 100μlE011230-1-AF350$485 / 100μlE011230-1-AF405M$485 / 100μlE011230-1-AF488$485 / 100μlE011230-1-AF514$485 / 100μlE011230-1-AF532$485 / 100μlE011230-1-AF555$485 / 100μlE011230-1-AF568$485 / 100μlE011230-1-AF405S$485 / 100μlE011230-1-AF405L$485 / 100μlE011230-1-AF546$485 / 100μlE011230-1-AF594$485 / 100μlE011230-1-AF610$485 / 100μlE011230-1-AF635$485 / 100μlE011230-1-AF647$485 / 100μlE011230-1-AF680$485 / 100μlE011230-1-AF700$485 / 100μlE011230-1-AF750$485 / 100μlE011230-1-AF790$485 / 100μlE011230-1-APC$485 / 100μlE011230-1-PE$485 / 100μlE011230-1-Cy3$485 / 100μlE011230-1-Cy5$485 / 100μlE011230-1-Cy5.5$485 / 100μlE011230-1-Cy7$485 / 100μlE011230-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.