World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
EGFR (Phospho-Tyr869) Antibody
$145/ 50μg/50μl
Specification
QuantityTotal: $145
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-YP-H-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityEGFR (phospho-Tyr869) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 869.
ReferencesInoue A, et al. (2005) PLoS Med; 2(1): e13
Sun H, et al. (2004) EMBO J; 23(1): 100-110
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94(7): 3250-3255
Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441
Sun H, et al. (2004) EMBO J; 23(1): 100-110
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94(7): 3250-3255
Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extract from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR (Ab-869) antibody (E021222, Lane1 and 2) and EGFR (phospho-Tyr869) antibody (E011229, Lane 3 and 4).
Conjugated Antibody
This product is also available conjugated. Format: E011229-1-Conjugate. Price: $485 / Size: 100μl.
E011229-1-FITC$485 / 100μlE011229-1-Biotin$485 / 100μlE011229-1-HRP$485 / 100μlE011229-1-AP$485 / 100μlE011229-1-AF350$485 / 100μlE011229-1-AF405M$485 / 100μlE011229-1-AF488$485 / 100μlE011229-1-AF514$485 / 100μlE011229-1-AF532$485 / 100μlE011229-1-AF555$485 / 100μlE011229-1-AF568$485 / 100μlE011229-1-AF405S$485 / 100μlE011229-1-AF405L$485 / 100μlE011229-1-AF546$485 / 100μlE011229-1-AF594$485 / 100μlE011229-1-AF610$485 / 100μlE011229-1-AF635$485 / 100μlE011229-1-AF647$485 / 100μlE011229-1-AF680$485 / 100μlE011229-1-AF700$485 / 100μlE011229-1-AF750$485 / 100μlE011229-1-AF790$485 / 100μlE011229-1-APC$485 / 100μlE011229-1-PE$485 / 100μlE011229-1-Cy3$485 / 100μlE011229-1-Cy5$485 / 100μlE011229-1-Cy5.5$485 / 100μlE011229-1-Cy7$485 / 100μlE011229-1-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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