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Primary Antibodies
4E-BP1 (Phospho-Thr45) Antibody
$145/ 50μg/50μl
Specification
QuantityTotal: $145
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
All Names
Altername4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I
All SitesHuman: Thr45 or Thr46; Mouse: Thr44; Rat: Thr44
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity4E-BP1 (phospho-Thr45) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
ReferencesGingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (phospho-Thr45) antibody (E011223).
Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min), using 4E-BP1 (Ab-45) antibody (E021216, Lane1 and 2) and 4E-BP1 (phospho-Thr45) antibody (E011223, Lane 3 and 4) .
Conjugated Antibody
This product is also available conjugated. Format: E011223-1-Conjugate. Price: $485 / Size: 100μl.
E011223-1-FITC$485 / 100μlE011223-1-Biotin$485 / 100μlE011223-1-HRP$485 / 100μlE011223-1-AP$485 / 100μlE011223-1-AF350$485 / 100μlE011223-1-AF405M$485 / 100μlE011223-1-AF488$485 / 100μlE011223-1-AF514$485 / 100μlE011223-1-AF532$485 / 100μlE011223-1-AF555$485 / 100μlE011223-1-AF568$485 / 100μlE011223-1-AF405S$485 / 100μlE011223-1-AF405L$485 / 100μlE011223-1-AF546$485 / 100μlE011223-1-AF594$485 / 100μlE011223-1-AF610$485 / 100μlE011223-1-AF635$485 / 100μlE011223-1-AF647$485 / 100μlE011223-1-AF680$485 / 100μlE011223-1-AF700$485 / 100μlE011223-1-AF750$485 / 100μlE011223-1-AF790$485 / 100μlE011223-1-APC$485 / 100μlE011223-1-PE$485 / 100μlE011223-1-Cy3$485 / 100μlE011223-1-Cy5$485 / 100μlE011223-1-Cy5.5$485 / 100μlE011223-1-Cy7$485 / 100μlE011223-1-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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