Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivitymTOR (phospho-Ser2448) antibody detects endogenous levels of mTOR only when phosphorylated at serine 2448.
ReferencesHolz MK, et,al. (2005) J Biol Chem ;280:26089-26093
Chiang GG, et,al. (2005) J Biol Chem ;280: 25485-25490
Mothe-Satney I, et,al. (2004) J Biol Chem ;279: 42628-42637
Bolster DR, et,al. (2003) J Physiol ;553:213-220.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells