World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
NFκB-p65 (Phospho-Ser529) Antibody
$145/ 50μg/50μl
Specification
QuantityTotal: $145
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
All Names
AlternameNF-KB p65, NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65
All SitesHuman: Ser529; Mouse: Ser529; Rat: Ser529
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityNF-κB p65 (phospho-Ser529) antibody detects endogenous levels of NF-κB p65 protein only when phosphorylated at serine 529.
ReferencesXu C, et al (2005) Oncogene:24(28): 4486-95.
McNulty SE, et al. (2004) Pigment Cell Res Apr; 17(2): 173-80.
Madrid LV,et al. (2001) J Biol Chem: 276(22): 18934-40.
Wang D, et al. (2000) J Biol Chem : 275(42): 32592-7.
McNulty SE, et al. (2004) Pigment Cell Res Apr; 17(2): 173-80.
Madrid LV,et al. (2001) J Biol Chem: 276(22): 18934-40.
Wang D, et al. (2000) J Biol Chem : 275(42): 32592-7.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-κB p65 (Phospho-Ser529) antibody (E011217).
Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-α (20ng/ml, 10min) using NF-κB p65 (Ab-529) antibody (E021210, Line 1 and 2) and NF-κB p65 (phospho-Ser529) antibody (E011217, Line 3 and 4).
Conjugated Antibody
This product is also available conjugated. Format: E011217-1-Conjugate. Price: $485 / Size: 100μl.
E011217-1-FITC$485 / 100μlE011217-1-Biotin$485 / 100μlE011217-1-HRP$485 / 100μlE011217-1-AP$485 / 100μlE011217-1-AF350$485 / 100μlE011217-1-AF405M$485 / 100μlE011217-1-AF488$485 / 100μlE011217-1-AF514$485 / 100μlE011217-1-AF532$485 / 100μlE011217-1-AF555$485 / 100μlE011217-1-AF568$485 / 100μlE011217-1-AF405S$485 / 100μlE011217-1-AF405L$485 / 100μlE011217-1-AF546$485 / 100μlE011217-1-AF594$485 / 100μlE011217-1-AF610$485 / 100μlE011217-1-AF635$485 / 100μlE011217-1-AF647$485 / 100μlE011217-1-AF680$485 / 100μlE011217-1-AF700$485 / 100μlE011217-1-AF750$485 / 100μlE011217-1-AF790$485 / 100μlE011217-1-APC$485 / 100μlE011217-1-PE$485 / 100μlE011217-1-Cy3$485 / 100μlE011217-1-Cy5$485 / 100μlE011217-1-Cy5.5$485 / 100μlE011217-1-Cy7$485 / 100μlE011217-1-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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