Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityFAK (phospho-Tyr397) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 397.
ReferencesParsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026.
Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells