Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 217 (I-D-SP-M-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityMEK1 (phospho-Ser217) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 217
ReferencesShen R, et al. (2002) Mol Cell Biol; 22(10): 3230-3236
Preisinger C, et al. (2005) EMBO J; 24(4): 753-765
Laine P, et al. (2000) Biochem J; 349(Pt 1): 19-25
Yaglom J, et al. (2003) Mol Cell Biol; 23(11): 3813-3824
Dahan S, et al. (2002) Infect Immun; 70(5): 2304-2310
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells