Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human
Raf1 around the phosphorylation site of serine 338 (R-D-SP-S-Y).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityRaf1 (phospho-Ser338) antibody detects endogenous levels of Raf1 only when
phosphorylated at serine 338.
ReferencesAdams DG,et al. (2005)J Biol Chem. 280(52):42644-54.
Miura S, et al. (2003) Biochem Biophys Res Commun. 306(4):924-9.
Noser JA,et al.(2007)Mol Ther.15(8):1531-6.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells