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Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody (E021200, Lane 1 and 2) and Ezrin (phospho-Thr566) antibody (E011202, Lane 3 and 4).

Primary Antibodies

Ezrin (Phospho-Thr566) Antibody

Name: Ezrin (Phospho-Thr566) Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E011202-1
Price: 145 USD
Availability: InStock

E011202-1Cite ID: EN0000379Datasheet COA MSDS

ReactivityHuman, Mouse, Rat

ApplicationsWB: 1:500~1:1000

Swiss-Prot No.P15311

$145/ 50μg/50μl

Specification

Quantity

Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (1)Documents

All Names

AlternameCytovillin, EZRI, VIL2, Villin 2, p81

All SitesHuman: Thr566 or Thr567; Mouse:Thr566 or Thr567; Rat: Thr567

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/StabilityStore at -20℃/1 year

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 566 (Y-K-TP-L-R).

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEzrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at threonine 566.

ReferencesZhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91
Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95
Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90
Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody (E021200, Lane 1 and 2) and Ezrin (phospho-Thr566) antibody (E011202, Lane 3 and 4).

Price145$

Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody (E021200, Lane 1 and 2) and Ezrin (phospho-Thr566) antibody (E011202, Lane 3 and 4).

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E011202-1-Conjugate. Price: $485 / Size: 100μl.

E011202-1-FITC$485 / 100μlE011202-1-Biotin$485 / 100μlE011202-1-HRP$485 / 100μlE011202-1-AP$485 / 100μlE011202-1-AF350$485 / 100μlE011202-1-AF405M$485 / 100μlE011202-1-AF488$485 / 100μlE011202-1-AF514$485 / 100μlE011202-1-AF532$485 / 100μlE011202-1-AF555$485 / 100μlE011202-1-AF568$485 / 100μlE011202-1-AF405S$485 / 100μlE011202-1-AF405L$485 / 100μlE011202-1-AF546$485 / 100μlE011202-1-AF594$485 / 100μlE011202-1-AF610$485 / 100μlE011202-1-AF635$485 / 100μlE011202-1-AF647$485 / 100μlE011202-1-AF680$485 / 100μlE011202-1-AF700$485 / 100μlE011202-1-AF750$485 / 100μlE011202-1-AF790$485 / 100μlE011202-1-APC$485 / 100μlE011202-1-PE$485 / 100μlE011202-1-Cy3$485 / 100μlE011202-1-Cy5$485 / 100μlE011202-1-Cy5.5$485 / 100μlE011202-1-Cy7$485 / 100μlE011202-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.