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Primary Antibodies

EGFR (Phospho-Thr678) Antibody

E011186-1Cite ID: EN0000355DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000
P00533
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEGFR (phospho-Thr678) antibody detects endogenous levels EGFR only when phosphorylated at threonine 678.
ReferencesDoherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94(7): 3250-3255
Noguchi T, et al. (1994) Mol Cell Biol; 14(10): 6674-6682
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR (Ab-678) antibody (E021193, Line 1 and 2) and EGFR (phospho-Thr678) antibody (E011186, Line 3 and 4).
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR (Ab-678) antibody (E021193, Line 1 and 2) and EGFR (phospho-Thr678) antibody (E011186, Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011186-1-Conjugate. Price: $485 / Size: 100μl.

E011186-1-FITC$485 / 100μlE011186-1-Biotin$485 / 100μlE011186-1-HRP$485 / 100μlE011186-1-AP$485 / 100μlE011186-1-AF350$485 / 100μlE011186-1-AF405M$485 / 100μlE011186-1-AF488$485 / 100μlE011186-1-AF514$485 / 100μlE011186-1-AF532$485 / 100μlE011186-1-AF555$485 / 100μlE011186-1-AF568$485 / 100μlE011186-1-AF405S$485 / 100μlE011186-1-AF405L$485 / 100μlE011186-1-AF546$485 / 100μlE011186-1-AF594$485 / 100μlE011186-1-AF610$485 / 100μlE011186-1-AF635$485 / 100μlE011186-1-AF647$485 / 100μlE011186-1-AF680$485 / 100μlE011186-1-AF700$485 / 100μlE011186-1-AF750$485 / 100μlE011186-1-AF790$485 / 100μlE011186-1-APC$485 / 100μlE011186-1-PE$485 / 100μlE011186-1-Cy3$485 / 100μlE011186-1-Cy5$485 / 100μlE011186-1-Cy5.5$485 / 100μlE011186-1-Cy7$485 / 100μlE011186-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.