Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityEGFR (phospho-Thr678) antibody detects endogenous levels EGFR only when phosphorylated at threonine 678.
ReferencesDoherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94(7): 3250-3255
Noguchi T, et al. (1994) Mol Cell Biol; 14(10): 6674-6682
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells