Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityAMPK1(Phospho-Thr174) Antibody detects endogenous levels of AMPK1 only when phosphorylated at threonine 174.
ReferencesHurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066
Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008
Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641
Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells