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Primary Antibodies

AMPK1 (Phospho-Thr174) Antibody

E011183-1Cite ID: EN0000349DatasheetCOAMSDS
Human, Mouse, Rat
IHC: 1:50-1:100
Q13131
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAMPK1(Phospho-Thr174) Antibody detects endogenous levels of AMPK1 only when phosphorylated at threonine 174.
ReferencesHurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066
Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008
Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641
Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPK1 (Phospho-Thr174) Antibody (E011183).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPK1 (Phospho-Thr174) Antibody (E011183).

Conjugated Antibody

This product is also available conjugated. Format: E011183-1-Conjugate. Price: $485 / Size: 100μl.

E011183-1-FITC$485 / 100μlE011183-1-Biotin$485 / 100μlE011183-1-HRP$485 / 100μlE011183-1-AP$485 / 100μlE011183-1-AF350$485 / 100μlE011183-1-AF405M$485 / 100μlE011183-1-AF488$485 / 100μlE011183-1-AF514$485 / 100μlE011183-1-AF532$485 / 100μlE011183-1-AF555$485 / 100μlE011183-1-AF568$485 / 100μlE011183-1-AF405S$485 / 100μlE011183-1-AF405L$485 / 100μlE011183-1-AF546$485 / 100μlE011183-1-AF594$485 / 100μlE011183-1-AF610$485 / 100μlE011183-1-AF635$485 / 100μlE011183-1-AF647$485 / 100μlE011183-1-AF680$485 / 100μlE011183-1-AF700$485 / 100μlE011183-1-AF750$485 / 100μlE011183-1-AF790$485 / 100μlE011183-1-APC$485 / 100μlE011183-1-PE$485 / 100μlE011183-1-Cy3$485 / 100μlE011183-1-Cy5$485 / 100μlE011183-1-Cy5.5$485 / 100μlE011183-1-Cy7$485 / 100μlE011183-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.