Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of threonine261 (A-K-TP-R-D) .
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
Specificty Sensitivity
Specificity/SensitivitySEK1/MKK4 (phospho-Thr261) antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at threonine 261.
ReferencesWang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001) J Biol Chem; 276(3): 1961-7.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells