Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PLCγ2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityPLCγ2 (phospho-Tyr753) antibody detects endogenous levels of PLCγ2 only when phosphorylated at tyrosine 753.
ReferencesKim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999
Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661
Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029
Rodriguez R, et al. (2003) Biochem J 374 : 269-280
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells