World supplier of antibodies, antigens and kits since 2007
Primary Antibodies

AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody

E011174-1Cite ID: EN0000333DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
Q13131
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G- SP-V-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAMPK1/AMPK2 (phospho-Ser485/Ser491) antibody detects endogenous levels of AMPK1/AMPK2 only when phosphorylated at serine 485 or serine 491.
ReferencesKim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody (E011174).
Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody (E011174).
Western blot analysis of extract from HeLa cells untreated or treated with heat shock (30min), using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody (E011174).
Western blot analysis of extract from HeLa cells untreated or treated with heat shock (30min), using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody (E011174).

Conjugated Antibody

This product is also available conjugated. Format: E011174-1-Conjugate. Price: $485 / Size: 100μl.

E011174-1-FITC$485 / 100μlE011174-1-Biotin$485 / 100μlE011174-1-HRP$485 / 100μlE011174-1-AP$485 / 100μlE011174-1-AF350$485 / 100μlE011174-1-AF405M$485 / 100μlE011174-1-AF488$485 / 100μlE011174-1-AF514$485 / 100μlE011174-1-AF532$485 / 100μlE011174-1-AF555$485 / 100μlE011174-1-AF568$485 / 100μlE011174-1-AF405S$485 / 100μlE011174-1-AF405L$485 / 100μlE011174-1-AF546$485 / 100μlE011174-1-AF594$485 / 100μlE011174-1-AF610$485 / 100μlE011174-1-AF635$485 / 100μlE011174-1-AF647$485 / 100μlE011174-1-AF680$485 / 100μlE011174-1-AF700$485 / 100μlE011174-1-AF750$485 / 100μlE011174-1-AF790$485 / 100μlE011174-1-APC$485 / 100μlE011174-1-PE$485 / 100μlE011174-1-Cy3$485 / 100μlE011174-1-Cy5$485 / 100μlE011174-1-Cy5.5$485 / 100μlE011174-1-Cy7$485 / 100μlE011174-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.